Free Testo, RIA CT, 96 tests
Radioimmunoassay for the Quantitative Determination of Free Testosterone in Human serum. Since the concentrations of I - FT and coated antibodies are constant, the advancing state of the equation depends on the concentration of FT. The amount of 125I - FT bound to the coated tube is inversely proportional to the concentration of FT in the sample.
Following the incubation, the tube is aspirated to remove excess unbound labelled T. Patient sample concentrations are read from a calibration curve.