Angiotensin I RIA, RIA CT, 100 tests
The Angiotensin I RIA serves for the quantitative determination of plasma renin activity (PRA) by the radioimmunoassay of the product of the reaction, angiotensin I.
The generation of angiotensin I is the result of the enzymatic cleavage of the renin substrate, angiotensinogen, in plasma samples in the presence of ACE inhibitor (ACE - Angiotensin-Converting Enzyme), an enzymatic inhibitor that blocks the conversion of angiotensin I to angiotensin II.
The immunoassay of angiotensin I is a radioimmunological competition assay.
Unknown samples, control, and calibrators are incubated in polyclonal antibody-coated tubes with 125I-labeled angiotensin I as tracer.
After incubation, the contents of the tubes are aspirated.
The bound radioactivity is then determined in a gamma counter.
A standard curve is established and unknown values are determined by interpolation from the standard curve.