Malaria Ab ELISA, 192 tests

The KAPDMA, Malaria Ab ELISA, allows the determination of antibodies (IgG, IgM or IgA) to Plasmodium species in human sera and plasma.

The kit is intended for the screening of blood units and the identification of people that came into contact with the protozoa and developed an immunological response.

Recombinant proteins representing immunodominant epitopes of Plasmodium species, are coated onto wells of a microplate. If antibodies specific to P.species (IgG, IgM or IgA) are present in the sample, they will form stable complexes with the recombinant antigens in the well.

Antigen-antibody complexes are then identified through the successive addition of (1) same biotinylated recombinant proteins specific to P.species and (2) horseradish peroxidase HRP Streptavidin conjugate.The hydrolytic activity of horseradish peroxidase allows for the quantification of these antibody-antigen complexes. Peroxidase substrate solution is then added.

During incubation, a blue colour will develop in proportion to the amount of anti P.species antibodies bound to the well, thus establishing their presence or absence in the sample. Wells containing samples negative for anti-P.species antibody remain colourless.

A stop solution is added to each well and the resulting yellow colour is read on a microplate reader at 450 nm.