Melatonin RIA, 200 tests

Melatonin RIA kit measures melatonin by a double-antibody radioimmunoassay based on the Kennaway G280 anti-melatonin antibody.

Reversed-phase column extracted samples and Controls and reconstituted Calibrators are incubated with the anti-melatonin antibody and 125I-melatonin.

125I-melatonin competes with melatonin present in samples, Calibrators and Controls.

After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction.

After aspiration of the unbound fraction, the antibody bound fraction of 125I-melatonin is counted.