Tg Ab, RIA CT, 96 tests
The present method is based on a competitive radioimmunoassay (RIA).
During the incubation, the monoclonal anti-Tg antibody on solid phase competes with the sample/calibrator anti-Tg autoantibodies for the specific sites of the Iodine125 labelled Tg (tracer). After aspiration and washing, the radioactivity in the tubes is measured in a gamma counter. The degree of binding will be inversely proportional to the sample anti-Tg autoantibody concentration.
The calibrators of RIAZENco TG Ab are calibrated against the TgAb international reference preparation MRC65/93.