Free Testo, ELISA, 96 tests

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in calibrators, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of free testosterone in the sample. A set of calibrators is used to plot a calibration curve from which the amount of free testosterone in patient samples and controls can be directly read. The DIAsource free testosterone kit utilizes a highly specific rabbit anti-testosterone polyclonal antibody at a low binding capacity (Keq x concentration) to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.

Catalog # KAPDB260
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 25 µL
Controls 2 levels
Range 0,1-60 pg/mL
Sensitivity 0,0188 pg/mL
Incubation 60/15 min. at 37°C
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact:

Documents - Download
Download "Instructions for Use" KAPDB260.pdf
Download "Material Safety Data Sheet" MSKAPDB260.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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