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ELISA & Instruments

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA).

ELISA Products by DIAsource

After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

DIAsource ImmunoAssays has a unique ELISA (>160 assays) product portfolio in endocrinology, auto-immunity and virology. Most recently the company made major investments in the development of antibodies and different assays for Vitamin D (RIA and ELISA).

DIAsource ImmunoAssays proposes a wide range of ELISA instruments including the Stratec GEMINI, an open ELISA platform. The Stratec GEMINI is a fully automated microplate processor for low throughput applications. The Stratec GEMINI and DIAsource ELISA assays, are the right combination for any ELISA measurement in private or public laboratories.

Coordinates

DIAsource ImmunoAssays SA
BE 0457.934.723
Rue du Bosquet, 2 | 1348 Louvain-la-Neuve - Belgium
Tel +32 10 84 99 11 | Fax +32 10 84 99 90

CLOSING DAYS 2019 : 1/01-22/04-01&30/05-10/06-21/07-15/08-01&11/11-25/12

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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