CBG, RIA CT, 96 tests
A fixed amount of I labelled CBG competes with the CBG to be measured present in the sample or in the calibrator for a fixed amount of anti-CBG antibody sites, which are bound to the goat anti mouse (GAM) antibodies immobilized to the wall of a polystyrene tube.
After 2 hours incubation at room temperature, an aspiration step terminates the competition reaction. The tubes are then washed with 2 ml of working wash solution and aspirated again. A calibration curve is plotted and the CBG concentrations of the samples are determined by dose interpolation from the calibration curve.