Syphilis Ab, ELISA, 96 tests

The kit is intended for the detection of anti-treponemal antibodies in a sample by means of a one-step sandwich type of the EIA method (i.e. a solid phase coated with specific antigens – antibody from the analysed sample – conjugate, i.e. specific antigens of Treponema pallidum conjugated with horseradish peroxidase).

Peroxidase activity is determined in the test by a substrate containing TMB.

Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific antibodies in the sample.