TSH-R Ab, ELISA, 96 tests

In the ELISA kit, TSHR autoantibodies in patient sera, calibrators and controls are allowed to interact with TSH receptors coated onto ELISA plate wells. After a 2 hour incubation, the samples are discarded leaving TRAb bound to the immobilised receptor. A human monoclonal autoantibody to the TSHR labelled with biotin (M22-biotin) is added in a second incubation step, where it interacts with immobilised TSH receptors which have not been blocked by bound TRAb. The amount of M22-biotin bound to the plate is then determined in a third incubation step by addition of streptavidin peroxidase, which binds specifically to biotin. Excess unbound streptavidin peroxidase is then washed away and addition of 3,3’,5,5’-tetramethylbenzidine (TMB) resulting in the formation of a blue colour. This reaction is stopped by addition of stop solution causing the well contents to turn from blue to yellow. The absorbance of the yellow reaction mixture at 450nm is then read using an ELISA plate reader. A lower absorbance indicates the presence of TRAb in a test sample (as TRAb inhibit the binding of M22-biotin to TSHR coated plate wells).

Catalog # KAPD4834
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 75 µL
Controls 2 levels
Range 0,4-30 U/L
Sensitivity 0,08 U/L
Incubation 2h/25 min./20 min./30 min.
Shelf Life (weeks) 48

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPD4834.pdf
Download "Material Safety Data Sheet" MSKAPD4834.pdf
Download catalogue "ELISA" CATE.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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