In the present kit, DIAsource has introduced a pre-treatment step in order to improve the clinical performance of the assay. It is well established that the binding proteins interfere with the radioimmunoassay for SM-C. The pre-treatment step used by DIAsource is the acid-ethanol procedure of Daughaday et al..A fixed amount of 125I labelled SM-C competes with the SM-C to be measured present in the sample or in the calibrator for a fixed amount of antibody sites being immobilized to the wall of a polystyrene tube. After an overnight incubation at 2-8°C, an aspiration step stops the competition reaction. The tubes are then washed with 3 ml of wash solution and aspirated again. A calibration curve is plotted and the SM-C concentrations of the samples are determined by dose interpolation from the calibration curve.
| Catalog # | KIP1589 |
| Format | RIA CT |
| Label | I125 |
| Size | 96 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 100 µL |
| Controls | 3 levels |
| Range | 17,5-1750 ng/mL |
| Sensitivity | 8,75 ng/mL |
| Incubation | Overnight at 2-8°C |
| Shelf Life (weeks) | 6 |
| Calibration | 1 ng = 1 ng of the 1st IS 91/554 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KIP1589.pdf |  |
| Download "Material Safety Data Sheet" | MSKIP1589.pdf | |
| Download catalogue "RIA" | CATR.pdf | |
| Download RIA Availability Calendar | CALRIA21d.pdf | |
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