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1,25(OH)2 Vitamin D, RIA CT, 48 tests

The DIAsource 1,25(OH)2 Vitamin D RIA is a competitive RIA assay, based on the Gold Standard extraction methodology.

The assay shows equal or superior sensitivity, precision and cross-reactivity profile to all other RIA assays in the market.

Only samples and controls, not the calibrators, are extracted with a mix of solvents and applied on cartridges to separate 1,25(OH)2 Vitamin-D from other Vitamin-D metabolites. After elution of samples and controls, the calibrators, samples and controls are incubated in coated tubes. A fixed amount of 125I labelled 1,25(OH)2 Vitamin D competes with the 1,25(OH)2 Vitamin D to be measured present in the sample or in the calibrator for a fixed amount of antibody sites immobilized on the wall of a polystyrene tube. After an overnight incubation at room temperature, an aspiration step terminates the competition reaction. The tubes are then washed with washing solution and aspirated. A calibration curve is plotted and the 1,25(OH)2 Vitamin D concentrations of the samples are determined by dose interpolation from the calibration curve.

A RUO version is available for US customers. Contact our customer services for more information.

Download our Technical Review on Performance of the DIAsource 1,25(OH)2 Vitamin D RIA assay in DEQAS and Instand Quality Control Programs.

Download our Technical Review on Technical Review 2015-05 - Performance of the DIAsource 1,25(OH)2 Vitamin D RIA assay in DEQAS Quality Control Program.

Download our Technical Review on Technical Review 2015-06 - Correlation of the DIAsource 1,25(OH)2 Vitamin D RIA assay with LC-MS.

Download our Technical Review on Technical Review 2015-07 - Evaluation of the DIAsource 25OH Vitamin D Total RIA assay according to the Vitamin D Standardization Program (VDSP).

Catalog # KIP1929
Format RIA CT
Label I125
Size 48 tests
Sample Type Serum, Plasma
Sample Volume 500 µL
Controls 2 levels
Range 6-430 pg/mL
Sensitivity 0,5 pg/mL
Incubation Overnight at RT
Shelf Life (weeks) 10

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KIP1929.pdf
Download catalogue "Vitamin D" CATV.pdf
Download RIA Availability Calendar CALRIA21d.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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