INS, ELISA, 96 tests

The DIAsource INS-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on breakable microtiterplates. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of insulin.Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human insulin – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the insulin concentration. A calibration curve is plotted and INS concentration in samples is determined by interpolation from the calibration curve.

Catalog # KAP1251
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 50 µL
Controls 2 levels
Range 5.1-250 µIU/mL
Sensitivity 0,17 µIU/mL
Incubation 45 min at RT
Shelf Life (weeks) 60
Calibration 1 µIU = 1 µIU of 2nd IRP 66/304
Comments New Reconstitution volume for controls

Due to local registration requirement, this product can not be sold in Canada, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1251.pdf
Download "Material Safety Data Sheet" MSKAP1251.pdf
Download catalogue "ELISA" CATE.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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