At first calibrators, controls and samples (serum or heparin plasma) are pre-treated to release 25OH Vitamin D2 and 25OH Vitamin D3 from Vitamin D Binding Protein (DBP). A fixed amount of 125I labelled 25OH Vitamin D competes with the 25OH Vitamin D2 and 25OH Vitamin D3 from treated samples, controls or calibrators, for a fixed amount of a specific monoclonal antibody sites immobilized to the lower and inner surface of plastic tubes. After 2 hours incubation at room temperature on a tube shaker, an aspiration step terminates the competition reaction. The tubes are then washed twice and aspirated again. A calibration curve is plotted and the total 25 OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.
| Catalog # | KIP1974 |
| Format | RIA CT |
| Label | I125 |
| Size | 384 tests |
| Sample Type | Serum |
| Sample Volume | 25 µL |
| Controls | 2 levels |
| Range | 10-100 ng/mL |
| Sensitivity | 1,63 ng/mL |
| Pretreatment | direct pretreatment |
| Incubation | 3 hours at room temperature |
| Shelf Life (weeks) | 8 |
- Gold Standard Diagnostics
- 2851 Spafford Street, Suite A
- CA 95618
- Davis
- United States
- Tel +1 530 759 8000
- Fax +1 530 759 8012
- info@gsdx.us
- www.gsdx.us
- IBL-America (Immuno-Biological Laboratories, Inc.)
- 8201 Central Ave NE
- MN 55432
- Minneapolis
- United States
- Tel +1 888 523 1246
- Fax +1 763 780 2988
- info@ibl-america.com
- www.ibl-america.com
Iodination Schedule 2015 :
| Download "Instructions for Use" | KIP1971.pdf |  |
| Download "Material Safety Data Sheet" | MSKIP1971.pdf | |
| Download brochure "25OH Vitamin D Total RIA Assay" | 25ohr.pdf | |
| Download Calendar | CAL2019.pdf | |
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