At first calibrators, controls and samples (serum or heparin plasma) are pre-treated to release 25OH Vitamin D2 and 25OH Vitamin D3 from Vitamin D Binding Protein (DBP). A fixed amount of 125I labelled 25OH Vitamin D competes with the 25OH Vitamin D2 and 25OH Vitamin D3 from treated samples, controls or calibrators, for a fixed amount of a specific monoclonal antibody sites immobilized to the lower and inner surface of plastic tubes. After 2 hours incubation at room temperature on a tube shaker, an aspiration step terminates the competition reaction. The tubes are then washed twice and aspirated again. A calibration curve is plotted and the total 25 OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.
Catalog # | KIP1974 |
Format | RIA CT |
Label | I125 |
Size | 384 tests |
Sample Type | Serum |
Sample Volume | 25 µL |
Controls | 2 levels |
Range | 10-100 ng/mL |
Sensitivity | 1,2 ng/mL |
Pretreatment | direct pretreatment |
Incubation | 3h at RT with shaking |
Shelf Life (weeks) | 8 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.