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ANCA, DIAspot, 24 tests

DIASpot ANCA+GBM IgG is an Immunodot kit intended for the detection in human sera of IgG autoantibodies against the Myeloperoxydase (MPO), Proteinase 3 (PR3) and Glomerular Basement Membrane/Collagen IV-α3 antigens. The test is based on the principle of an Enzyme Immunoassay. The test strip is composed of a membrane fixed on a plastic support. During test procedure, the strips are incubated with diluted patients’ sera. Human antibodies, if present, bind to the corresponding specific antigen(s) on the membrane. Unbound or excess antibodies are removed by washing and AP-conjugated goat antibodies against human IgG are added to the strips. This enzyme conjugate binds to the antigen-antibody complexes. After a second washing step to remove excess conjugate, substrate solution is added. Enzyme activity, if present, leads to the development of purple dots on the membrane pads. The intensity of the coloration is directly proportional to the amount of antibody present in the sample

Format DIAspot
Size 24 tests
Sample Volume 10 µL
Controls 2 levels
Incubation 64 min
Shelf Life (weeks) 48

Due to local registration requirement, this product can not be sold in Italy, Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPDTANCAG.pdf
Download "Material Safety Data Sheet" MSKAPDTANCAG.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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