IGF-1, RIA CT, 96 tests

In the present kit, DIAsource has introduced a pre-treatment step in order to improve the clinical performance of the assay. It is well established that the binding proteins interfere with the radioimmunoassay for IGF-I. A fixed amount of 125I labelled IGF-I compete with the IGF-I to be measured present in the sample or in the calibrator for a fixed amount of antibody sites immobilized on the wall of a polystyrene tube. After 2 hours incubation at room temperature with shaking , an aspiration step terminates the competition reaction. The tubes are then washed with 2 ml of washing solution and aspirated. A calibration curve is plotted and the IGF-I concentrations of the samples are determined by dose interpolation from the calibration curve