ACTH, IRMA, 96 tests, RUO (Research Use Only)
The DIAsource ACTH-IRMA is a two-step immunoradiometric assay based on coated-tube separation.
It allows the determination of intact human adrenocorticotropic hormone (ACTH) in EDTA plasma.
Monoclonal antibodies specific to the 1-24 ACTH fragment (N-terminal fragment) are attached to the lower and inner surface of the plastic tubes.
Calibrators or samples are added to the tubes.
After 2 hour incubation, washing removes the occasional excess of antigen, mid-regional and C-terminal fragments.
125I labelled polyclonal antibodies specific to the 24-39 ACTH fragment (Cterminal fragment) are added.
After 1 hour incubation and washing the remaining radioactivity bound to the tube reflects the intact ACTH concentration.