Reverse T3, RIA, 125 tests, RUO (Research Use Only)
The kit has been designed for quantitative determination, by RIA, of reverse T3 in serum, plasma or amniotic samples, without any preliminary treatment of the samples.
Antigen, whether from standards or from samples competes with radioactive tracer (I125-RT3) for the binding sites of antibody.
After incubation, the amount of tracer bound to antibody will be inversely proportional to the amount of antigen present in standards or in samples. B/F separation is performed by addition of polyethylene glycol and the immunocomplex is collected by centrifugation.