Pancreatic Polypeptide (PP), RIA, 100 tests

The intended use of these reagents is for assay of PP in human serum.

PP in serum is assayed without extraction by a competetive radioimmunoassay using a rabbit antiserum raised against bovine PP.

PP in standards and samples compete with 125I-labelled human PP in binding to the antibodies. 125I-PP binds in a reverse proportion to the concentration of PP in standards and samples.

Antibody-bound 125I-PP is separated from the unbound fraction using the double antibody-polyethyleneglycol precipitation technique.

The radioactivity of the precipitates is measured.

Human, synthetic PP is used for standardization