Vasoactive Intestinal Peptide (VIP), RIA, 100 tests

VIP is analysed by the competitive radioimmunoassay using antibodies to a VIP-albumin conjugate.

VIP in standards and samples compete with 125I-labelled VIP in binding to the antibodies.

125I-VIP binds in a reverse proportion to the concentration of VIP in standards and samples.

In order to increase the sensitivity of the assay a sequential incubation is performed.

Antibody-bound 125I-VIP is separated from the unbound fraction using the double antibody polyethylene glycol precipitation technique.

The radioactivity of the precipitates is measured.

The antiserum used in this kit is directed to the C-terminal part of the VIP molecule.