E2-ELISA is an enzyme immunoassay performed in a microtiter plate. A fixed amount of estradiol labeled with horseradish peroxidase (HRP) competes with unlabeled estradiol present in calibrators or samples for a limited number of binding sites of a specific antibody. The E2-HRP-antibody complex is simultaneously fixed on the wells of the microtiter plate coated with an excess of anti-rabbit-gammaglobulins. Neither extraction nor chromatography are required due to the high specificity of the antibody. After 2 hours incubation at room temperature the microtiter plate is washed to stop the competition reaction. The substrate solution (tetramethylbenzidine (TMB) – H202) is added and incubated for 30 minutes. The reaction is stopped with H2S04 and the absorbance is measured at the appropriate wavelength. The amount of substrate turnover is inversely proportional to the estradiol concentration in the sample. A calibration curve is plotted and estradiol concentrations in samples are determined by interpolation from the calibration curve.
| Catalog # | KAP0621 |
| Format | ELISA |
| Label | HRP |
| Size | 96 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 50 µL |
| Controls | 2 levels |
| Range | 13-935 pg/mL |
| Sensitivity | 5 pg/mL |
| Incubation | 2,5 h at RT with shaking |
| Shelf Life (weeks) | 60 |
Due to local registration requirement, this product can not be sold in Canada, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KAP0621.pdf |  |
| Download "Material Safety Data Sheet" | MSKAP0621.pdf | |
| Download catalogue "ELISA" |
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