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E2, ELISA, 96 tests, For Research Use Only

E2-ELISA is an enzyme immunoassay performed in a microtiter plate. A fixed amount of estradiol labeled with horseradish peroxidase (HRP) competes with unlabeled estradiol present in calibrators or samples for a limited number of binding sites of a specific antibody. The E2-HRP-antibody complex is simultaneously fixed on the wells of the microtiter plate coated with an excess of anti-rabbit-gammaglobulins. Neither extraction nor chromatography are required due to the high specificity of the antibody. After 2 hours incubation at room temperature the microtiter plate is washed to stop the competition reaction. The substrate solution (tetramethylbenzidine (TMB) – H202) is added and incubated for 30 minutes. The reaction is stopped with H2S04 and the absorbance is measured at the appropriate wavelength. The amount of substrate turnover is inversely proportional to the estradiol concentration in the sample. A calibration curve is plotted and estradiol concentrations in samples are determined by interpolation from the calibration curve.

Catalog # KAP0621
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 50 µL
Controls 2 levels
Range 13-935 pg/mL
Sensitivity 5 pg/mL
Incubation 2,5 h at RT with shaking
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP0621.pdf
Download "Material Safety Data Sheet" MSKAP0621.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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