DIAsource hPTH-120 min-IRMA is a two-step immunoradiometric assay based on coated-tube separation. It allows the determination of intact human PTH (hPTH) in serum. Goat antibodies specific to the 1-34 hPTH fragment (N-terminal fragment) are attached to the lower and inner surface of the plastic tubes. Calibrators or samples are added to the tubes. After 1 hour incubation, washing removes the occasional excess of antigen, mid-regional and C-terminal fragments. 125I labelled monoclonal antibodies specific to the 44-68 hPTH fragment are added. After 1 hour incubation and washing the remaining radioactivity bound to the tube reflects the intact h-PTH concentration. This two-step IRMA is highly specific of the intact h-PTH and does not cross react with active and inactive fragments even at high concentrations as suggested by HACKENG and al.
| Catalog # | KIR1491 |
| Format | IRMA |
| Label | I125 |
| Size | 96 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 300 µL |
| Controls | 2 levels |
| Range | 13-1562 pg/mL |
| Sensitivity | 1,7 pg/mL |
| Incubation | 2h at RT with shaking |
| Shelf Life (weeks) | 9 |
| Calibration | 1 pg = 1 pg of NIBSC 95/646 |
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KIP1491.pdf |  |
| Download "Material Safety Data Sheet" | MSKIP1491.pdf | |
| Download catalogue "RIA" | ||
| Download RIA Availability Calendar |
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