The kit is intended for the detection of anti-treponemal IgM antibodies in a sample by means of a capture type of the EIA method (i.e. a solid phase coated with monoclonal antibody to human IgM immunoglobulins – antibody from the analysed sample – specific antigens labelled with peroxidase). Specific antigens of Treponema pallidum conjugated with horseradish peroxidase are used for the detection of antibodies. Peroxidase activity is determined in the test by a substrate containing TMB. Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific antibodies in the sample.
Catalog # | TpM096 |
Format | ELISA |
Label | HRP |
Size | 96 tests |
Sample Type | Serum, Plasma |
Sample Volume | 10 µL |
Controls | 3 levels |
Incubation | 30/30/30 min. at 37°C |
Shelf Life (weeks) | 60 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.