Home > IVD Products > ImmunoAssays > Infectious Diseases > Hepatitis B (HBV) > HBV, Anti-HBc Total, ELISA, 96 tests

HBV, Anti-HBc Total, ELISA, 96 tests

ANTI-HBc TOTAL ELISA is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immune assay) - based on a competitive principle. The solid phase of the microtiter plate is made of polystyrene wells coated with HBcAg and the liquid phase of human peroxidase conjugated Anti-HBc.When a serum or plasma specimen containing Anti-HBc is added to the HBcAg-coated wells together with the human peroxidase conjugated Anti-HBc and incubated, a competition will take place for the binding to the HBcAg on the wells. (HBcAg)-(Anti-HBc • peroxidase) complex and/or (HBcAg)-(Anti-HBc) complex will form on the wells. After washing of the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. Due to the competitive principle a color develops inversely proportional to the amount of Anti-HBc bound to HBcAg deriving from the specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm.

Catalog # KAPG4CBE3
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 50 µL
Controls 2 levels
Sensitivity 1,869 PEI Units/ml
Incubation 60 min/30 min
Calibration PEI Anti-HBc Total Reference Material

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPG4CBE3.pdf
Download "Material Safety Data Sheet" MSKAPG4CBE3.pdf
Download catalogue "ELISA" CATE.pdf
Bridging Science And Diagnostics
Choose my country

DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

Please select your country to see the most accurate content available in your area.