ANTI-HBc TOTAL ELISA is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immune assay) - based on a competitive principle. The solid phase of the microtiter plate is made of polystyrene wells coated with HBcAg and the liquid phase of human peroxidase conjugated Anti-HBc.When a serum or plasma specimen containing Anti-HBc is added to the HBcAg-coated wells together with the human peroxidase conjugated Anti-HBc and incubated, a competition will take place for the binding to the HBcAg on the wells. (HBcAg)-(Anti-HBc • peroxidase) complex and/or (HBcAg)-(Anti-HBc) complex will form on the wells. After washing of the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. Due to the competitive principle a color develops inversely proportional to the amount of Anti-HBc bound to HBcAg deriving from the specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm.
| Catalog # | KAPG4CBE3 |
| Format | ELISA |
| Label | HRP |
| Size | 96 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 50 µL |
| Controls | 2 levels |
| Sensitivity | 1,869 PEI Units/ml |
| Incubation | 60 min/30 min |
| Calibration | PEI Anti-HBc Total Reference Material |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KAPG4CBE3.pdf |  |
| Download "Material Safety Data Sheet" | MSKAPG4CBE3.pdf | |
| Download catalogue "ELISA" |
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