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HBV, Anti-HBc IgM, ELISA, 96 tests

ANTI-HBc IgM ELISA is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immunosorbent assay) –-based on the principle of “Anti-HBc IgM”. The solid phase of the microtiter plate is made of polystyrene wells coated with anti-human IgM. When a serum or plasma specimen containing Anti-HBc IgM is added to the Anti-human IgM-coated wells and incubated, IgM antibodies present in the specimen bind to the Anti-h IgM on the wells. After addition of an HBcAgcontaining reagent and a solution containing peroxidase-conjugated anti-HBc a further incubation takes place, during which (Anti-h IgM) • (Anti-HBc IgM) •(HBcAg) • (Anti-HBc• peroxidase) complex is formed on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells andincubated. If Anti-HBc IgM is present in the specimen, after washing, the activity of peroxidase on the wells reflects the content of anti-HBc IgM in a specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid.The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm

Catalog # KAPG4CME3
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 5 µL
Controls 2 levels
Sensitivity 2,5 PEI Units/ml
Incubation 60 min+30 min+30min
Calibration PEI Anti-HBc IgM Reference Material

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPG4CME3.pdf
Download "Material Safety Data Sheet" MSKAPG4CME3.pdf
Download catalogue "ELISA" CATE.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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