HBV, Anti-HBc IgM, ELISA, 96 tests

ANTI-HBc IgM ELISA is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immunosorbent assay) –-based on the principle of “Anti-HBc IgM”. The solid phase of the microtiter plate is made of polystyrene wells coated with anti-human IgM. When a serum or plasma specimen containing Anti-HBc IgM is added to the Anti-human IgM-coated wells and incubated, IgM antibodies present in the specimen bind to the Anti-h IgM on the wells. After addition of an HBcAgcontaining reagent and a solution containing peroxidase-conjugated anti-HBc a further incubation takes place, during which (Anti-h IgM) • (Anti-HBc IgM) •(HBcAg) • (Anti-HBc• peroxidase) complex is formed on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells andincubated. If Anti-HBc IgM is present in the specimen, after washing, the activity of peroxidase on the wells reflects the content of anti-HBc IgM in a specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid.The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm

• Technical spec.

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download

Download "Instructions for Use"	KAPG4CME3.pdf
Download "Material Safety Data Sheet"	MSKAPG4CME3.pdf
Download catalogue "ELISA"