IGFBP-3, ELISA, 96 tests

The DIAsource IGFBP3-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplates. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human IGFBP3– MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the human IGFBP3 concentration.

Catalog # KAP1171
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 10 µL
Controls 2 levels
Range 460-16070 ng/mL
Sensitivity 10 ng/mL
Incubation 2,5 hours at room temperature
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: RA@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1171.pdf
Download "Material Safety Data Sheet" MSKAP1171.pdf
Download brochure "Growth Factors" CATE.pdf
Coordinates

DIAsource ImmunoAssays SA
BE 0457.934.723
Rue du Bosquet, 2 | 1348 Louvain-la-Neuve - Belgium
Tel +32 10 84 99 11 | Fax +32 10 84 99 90

CLOSING DAYS 2019 : 1/01-22/04-01&30/05-10/06-21/07-15/08-01&11/11-25/12

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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