This enzyme immunoassay kit is suited for measuring IGFBP-1 in human serum or Heparin-plasma or in other body fluids, for example amnion fluid, mother milk, urine or saliva, as for diagnostic and scientific purposes. It is also suited to quantitate IGFBP-1 in cell culture media. The DIAsource IGFBP-1 Elisa KAPME01 is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP-1 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-IGFBP-1-Antibody binds in turn to the immobilised IGFBP-1. Finally, the bound peroxidase catalyses the substrate reaction resulting in a colored product. Therefore colour intensity is highly specific and quantitatively depending on the IGFBP-1-level of the samples.
| Catalog # | KAPME01 |
| Format | ELISA |
| Label | HRP |
| Size | 96 tests |
| Sample Type | Serum, Plasma, Amniotic fluid |
| Sample Volume | 20 µL |
| Controls | 2 levels |
| Range | 0,1-8 ng/mL |
| Sensitivity | 0,055 ng/mL |
| Incubation | 1,75 h at RT with shaking 350 rpm |
| Shelf Life (weeks) | 60 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KAPME01.pdf |  |
| Download "Material Safety Data Sheet" | MSKAPME01.pdf | |
| Download catalogue "ELISA" |
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