hGH, ELISA, 96 tests

The DIAsource HGH-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on a microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 –hGH – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. The Chromogenic Solution (TMB – H2O2) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the hGH concentration.

Catalog # KAP1081
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 50 µL
Controls 2 levels
Range 0,45-98 µIU/mL
Sensitivity 0,17 µIU/mL
Incubation 1h at RT
Calibration 1 µIU = 1 µIU NIBSC 98/574

For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1081.pdf
Download "Material Safety Data Sheet" MSKAP1081.pdf
Download catalogue "ELISA" CATEv2201.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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