DHT, RIA

The procedure follows the basic principle of radioimmunoassay where there is competition between the radioactive and non-radioactive antigen for a fixed number of antibody binding sites.

The amount of (I-125)-labeled analyte bound to the antibody is inversely proportional to the concentration of unlabeled analyte present.

The separation of free and bound antigen is achieved by using a double antibody system.

Clinical applications of 5α-Dihydrotestosterone level measurement : Measurement of DHT concentrations can be complicated by antibody cross-reactivity to testosterone. The DHT Double Antibody radioimmunoassay utilizes a sample oxydation / extraction procedure to remove most of the testosterone, coupled with a specific immunoassay for DHT.

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For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.

Technical Specification

Ref.: KIPI9900
Regulatory Status: CE-IVD
Format: RIA
Label: I125
Size: 100 tests
Sample Type: Serum, Plasma
Sample Volume: 100 µL of extracted sample
Controls: 2 levels
Range: 25-2500 pg/mL
Sensitivity: 20 pg/mL
Pretreatment: 300 µL of sample/ 40 min.
Incubation: 30min at 37°C + 20min + 20min centrifugation
Shelf Life (weeks): 12

For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.