The procedure follows the basic principle of radioimmunoassay where there is competition between the radioactive and non-radioactive antigen for a fixed number of antibody binding sites. The amount of (I-125)-labeled analyte bound to the antibody is inversely proportional to the concentration of unlabeled analyte present. The separation of free and bound antigen is achieved by using a double antibody system.
Catalog # | KIPI9900 |
Format | RIA |
Label | I125 |
Size | 100 tests |
Sample Type | Serum, Plasma |
Sample Volume | 100 µL of extracted sample |
Controls | 2 levels |
Range | 25-2500 pg/mL |
Sensitivity | 20 pg/mL |
Pretreatment | 300 µL of sample/ 40 min. |
Incubation | 30min at 37°C + 20min + 20min centrifugation |
Shelf Life (weeks) | 12 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.