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Sperm-Ab, ELISA, 96 tests

The anti-spermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from DIAsource is a solid-phase sandwich enzymeimmunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum.The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and calibrators are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’-tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength >550 nm is recommended.

Catalog # KAPD1826
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 5 µL
Controls 1 level
Range 31-250 U/mL
Incubation 2h at 37 °C and 30 min at RT
Shelf Life (weeks) 48

Due to local registration requirement, this product can not be sold in Canada, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPD1826.pdf
Download "Material Safety Data Sheet" MSKAPD1826.pdf
Download catalogue "ELISA" CATE.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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