Free ß-hCG, ELISA

The DRG Free β-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.

The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on a Free β-hCG molecule.

An aliquot of patient sample containing endogenous Free β-hCG is incubated in the coated well with enzyme conjugate, which is an anti-β-HCG antibody [rabbit] conjugated with horseradish peroxidase.

After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Free β-HCG in the sample.

Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Free β-HCG in the patient sample.