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Leptin,ELISA, 96 tests

The Leptin ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the leptin molecule. During the first incubation, leptin in the added sample binds to the immobilized antibody. Unbound sample material is washed off. In a second incubation added antiserum, which contains biotinylated anti-leptin antibody, forms a sandwich complex with the bound leptin on the microtiter wells. Afterwards, the unbound antiserum is washed off and streptavidin peroxidase complex (Enzyme Complex) is added for detection of the biotin molecules. After a washing step to remove all unbound substances, the solid phase is incubated with the substrate solution. The colorimetric reaction is stopped by addition of stop solution, and optical density (OD) of the resulting yellow product is measured. The intensity of color is proportional to the concentration of the analyte in the sample. A calibration curve is constructed by plotting OD values against concentrations of calibrators, and concentrations of unknown samples are determined using this calibration curve.

Catalog # KAPD2395
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Li-heparin Plasma
Sample Volume 15 µL
Controls 2 levels
Range 0,7 ng/mL - 100 ng/mL
Sensitivity 0,7 ng/mL
Incubation 3h at RT + 15 min at RT
Shelf Life (weeks) 52
Calibration WHO International Standard NIBSC 97/594

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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