Radioimmunoassay (RIA) is the term applied to the measurement of the concentration of antigen molecules using a radioactive label that quantitates the amount of antigen (i.e., hormone) by determination of the extent to which it combines with its antibody. In the assay, a limited amount of specific antibody (Ab) is reacted with the corresponding hormone (*H) labeled with a radioisotope. Upon addition of an increasing amount of the hormone (H), a correspondingly decreasing fraction of *H added is bound to the antibody. After separation of the bound from the free *H by various means, the amount of radioactivity in one or both of these two fractions is evaluated and used to construct a standard curve against which the unknown samples are measured.
Catalog # | 07-152101 |
Format | RIA |
Label | I125 |
Size | 125 tests |
Sample Type | EDTA Plasma |
Sample Volume | 200 µL |
Controls | 2 levels |
Range | 25-2000 pg/mL |
Incubation | 6h/16h at 4°C |
Shelf Life (weeks) | 5 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.