Renin,ELISA, 96 tests

The Renin ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the human active Renin molecule. An aliquot of patient sample containing endogenous Renin is incubated in the coated well together with Assay Buffer. After incubation, unbound components are washed off. Finally, Enzyme Conjugate, which is a monoclonal anti-Renin antibody conjugated with horseradish peroxidase, is added, and after incubation, unbound enzyme conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Renin in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of active Renin in the patient sample.

Catalog # KAPD5125
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, EDTA Plasma
Sample Volume 50 µL
Controls 2 levels
Range 0,80 - 128 pg/mL
Sensitivity 0,80 pg/mL
Incubation 3h at RT with shaking + 15 min at RT
Shelf Life (weeks) 52
Calibration WHO 1st International Standard for Renin 68/356

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Documents - Download
Download "Instructions for Use" KAPD5125.pdf
Download catalogue "ELISA" CATEv2201.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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