The DIAsource ACTH-IRMA is a two-step immunoradiometric assay based on coated-tube separation. It allows the determination of intact human adrenocorticotropic hormone (ACTH) in EDTA plasma. Monoclonal antibodies specific to the 1-24 ACTH fragment (N-terminal fragment) are attached to the lower and inner surface of the plastic tubes. Calibrators or samples are added to the tubes. After 2 hour incubation, washing removes the occasional excess of antigen, mid-regional and C-terminal fragments. 125I labelled polyclonal antibodies specific to the 24-39 ACTH fragment (Cterminal fragment) are added. After 1 hour incubation and washing the remaining radioactivity bound to the tube reflects the intact ACTH concentration.
Catalog # | KIP0061 |
Format | IRMA |
Label | I125 |
Size | 96 tests |
Sample Type | EDTA Plasma |
Sample Volume | 200 µL |
Controls | 2 levels |
Range | 9,6-1932 pg/mL |
Sensitivity | 1,16 pg/mL |
Incubation | 3h at RT with shaking |
Shelf Life (weeks) | 7 |
Calibration | 1pg = 1pg of NIBSC 74/555 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.