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CanAg ProGRP, ELISA, 96 tests

The CanAg ProGRP EIA is a solid-phase, one-step, non-competitive immunoassay based on antibodies specific for different epitopes specifically expressed in ProGRP. Calibrators, controls or unknown samples are incubated together with affinity purified biotinylated Anti-ProGRP polyclonal antibody and horseradish peroxidase (HRP) labelled Anti-ProGRP Monoclonal antibody E146 in Streptavidin coated microtiter strips. After washing, buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetra-methylbenzidine) is added to each well and the enzyme reaction is allowed to proceed. During the enzyme reaction a blue colour will develop if antigen is present. The intensity of the colour is proportional to the amount of ProGRP present in the samples. The colour intensity is determined in a microplate spectrophotometer at 450 nm after addition of Stop Solution. Calibration curves are constructed for each assay by plotting absorbance value versus the concentration for each calibrator. The ProGRP concentrations of unknown samples are then read from the calibration curve.

Catalog # 220-10
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 50 µL
Controls 2 levels
Incubation 2h/30 min.
Shelf Life (weeks) 60
Comments For Research Use Only

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" RV22010.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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