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Free PSA, IRMA, 100 tests

The technology uses two high affinity monoclonal antibodies in an immunoradiometric assay (IRMA) system. The 125I labelled signal-antibody binds to an epitope of the fPSA molecule spatially different from that recognized by the biotincapture- antibody. The two antibodies react simultaneously with the antigen present in standards or samples, which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. During a 2-hour incubation period with shaking immuno-complex is immobilized to the reactive surface of streptavidin coated test tubes. Reaction mixture is then discarded, test tubes washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of fPSA, the unknown concentration of fPSA in patient samples can determined.

Catalog # RK-85CT
Format IRMA
Label I125
Size 100 tests
Sample Type Serum
Sample Volume 50 µL
Controls 2 levels
Range 0,3-30 ng/mL
Sensitivity 0,03 ng/mL
Incubation 2h at RT with shaking
Shelf Life (weeks) 5

For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use"
Download catalogue "RIA" CATRv2201.pdf
Download RIA Availability Calendar
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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