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25OH Vitamin D3, RIA CT, 96 tests

The DIAsource 25OH Vitamin D3 RIA is a competitive RIA assay, specific for the D3 form of 25OH Vitamin D.

The assay is calibrated to the ID-LC-MS/MS reference method developed by Prof. Dr. Linda THIENPONT from the Ghent University in Belgium, as encompassed in the Vitamin D Standardization Program initiative. It shows a high correlation with other commercially available LC-MS/MS and RIA assays.

At first calibrators, controls and samples (serum or plasma) are extracted with acetonitrile. A fixed amount of 125I labelled 25OH Vitamin D3 competes with the 25OH Vitamin D3 from either extracted samples, controls or calibrators for a fixed amount of specific antibody sites immobilized to the lower and inner surface of plastic tubes.

After 2 hours incubation at room temperature, an aspiration step stops the competition reaction. The tubes are then washed with 3 ml washing solution and counted in a gamma counter.

A RUO version is available for US customers. Contact our customer services for more information.

Catalog # KIP1961
Format RIA CT
Label I125
Size 96 tests
Sample Type Serum, Heparin Plasma
Sample Volume 100 µL
Controls 2 levels
Range 4-145 ng/mL
Sensitivity 1,2 ng/mL
Incubation 2h at RT with shaking
Shelf Life (weeks) 10

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KIP1961.pdf
Download "Material Safety Data Sheet" MSKIP1961.pdf
Download catalogue "RIA" CATR.pdf
Download RIA Availability Calendar CALRIA21d.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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