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Normetanephrine, Urine Fastrack ELISA, 96 tests

During the sample preparation Normetanephrine (Normetadrenaline) is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Catalog # KAPL10-0400
Format ELISA
Label HRP
Size 96 tests
Sample Type Urine
Sample Volume 10 µL
Controls 2 levels
Range 40-4000 ng/mL
Sensitivity 20 ng/mL
Incubation 3,5 hours at RT with shaking
Shelf Life (weeks) 60
Documents - Download
Download "Instructions for Use" KAPL10-0400.pdf
Download "Material Safety Data Sheet" MSKAPL10-0400.pdf
Download brochure "Biogenic Amines I" CATE.pdf

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CLOSING DAYS 2019 : 1/01-22/04-01&30/05-10/06-21/07-15/08-01&11/11-25/12

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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