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Noradrenaline, ELISA, 96 tests

Noradrenaline (Norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte concentrations compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Catalog # KAPL10-0200
Format ELISA
Label HRP
Size 96 tests
Sample Type EDTA Plasma, Urine
Sample Volume 300 µL for Plasma /10 µL for Urine
Controls 2 levels
Range 93-33333 pg/mL for Plasma / 2,5-1000 ng/mL for Urine
Sensitivity 36 pg/mL for Plasma / 1,7 ng/mL for Urine
Incubation 4.5 h at RT with shaking 600 rpm
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPL10-0200.pdf
Download "Material Safety Data Sheet" MSKAPL10-0200.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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