Melatonin RIA kit measures melatonin by a double-antibody radioimmunoassay based on the Kennaway G280 anti-melatonin antibody. Reversed-phase column extracted samples and Controls and reconstituted Calibrators are incubated with the anti-melatonin antibody and 125I-melatonin. 125I-melatonin competes with melatonin present in samples, Calibrators and Controls. After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction. After aspiration of the unbound fraction, the antibody bound fraction of 125I-melatonin is counted.
Catalog # | RK-MEL2 |
Format | RIA |
Label | I125 |
Size | 200 tests |
Sample Type | Serum, Plasma, Urine |
Sample Volume | 400 µl of extracted sample |
Controls | 2 levels |
Sensitivity | 1 ml of sample for extraction on columns |
Incubation | Overnight at 2-8°C + 15min at 2-8°C |
Due to local registration requirement, this product can not be sold in United States, Australia, South Africa – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.