Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
| Catalog # | KAPL10-1000 |
| Format | ELISA |
| Label | HRP |
| Size | 96 tests |
| Sample Type | EDTA Plasma, Urine |
| Sample Volume | 25 µL for Plasma /10 µL for Urine |
| Controls | 2 levels |
| Range | 0,12 -50 ng/ml for Plasma / 0,3-125 ng/ml for Urine |
| Sensitivity | 0,18 ng/mL for Plasma / 0,22 ng/ml for Urine |
| Incubation | Overnight 2-8°C or 4,5 hours at RT with shaking 600 rpm |
| Shelf Life (weeks) | 60 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KAPL10-1000.pdf |  |
| Download "Material Safety Data Sheet" | MSKAPL10-1000.pdf | |
| Download catalogue "ELISA" | CATE.pdf | |
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