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Nephrines, Plasma Fastrack ELISA, 2 x 96 tests

First, the plasma proteins are removed by precipitation. After this Metanephrine (Metadrenaline) and Normetanephrine Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

Catalog # KAPL10-1400
Format ELISA
Label HRP
Size 2 x 96 tests
Sample Type EDTA Plasma
Sample Volume 200 µL
Controls 2 levels
Range see Metanephrine and Normetanephrine Plasma ELISA
Sensitivity see Metanephrine and Normetanephrine Plasma ELISA
Incubation see Metanephrine and Normetanephrine Plasma EIA
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPL10-1400.pdf
Download "Material Safety Data Sheet" MSKAPL10-1400.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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