ELISA & Instruments

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA).

ELISA Products by DIAsource

After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

DIAsource ImmunoAssays has a unique ELISA (>160 assays) product portfolio in endocrinology, auto-immunity and virology. Most recently the company made major investments in the development of antibodies and different assays for Vitamin D (RIA and ELISA).

DIAsource ImmunoAssays proposes a wide range of ELISA instruments including the Stratec GEMINI, an open ELISA platform. The Stratec GEMINI is a fully automated microplate processor for low throughput applications. The Stratec GEMINI and DIAsource ELISA assays, are the right combination for any ELISA measurement in private or public laboratories.

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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