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IGF-1, ELISA, 96 tests, For Research Use Only

The DIAsource IGF1-ELISA is a solid phase Enzyme Amplified SensitivityImmunoassay performed on polystyrene microtiterplates.In the present kit, DIAsource has introduced a pre-treatment step in order toimprove the clinical performance of the assay. It is well established that the binding proteins interfere with the ELISA assay for IGF1.The pre-treatment step used by DIAsource is the acid-ethanol procedure of Daughaday et al . A fixed amount of IGF1-labelled with horseradish peroxidase (HRP), compete with unlabelled IGF1 present in the calibrators ,controls and samples for a limited number of binding sites on a specific antibody. After 1 hour incubation at room temperature, the microtiterplate is washed to stop the competition reaction. . The Chromogenic solution (TMB ) is added and incubated for 15 min. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is inversely proportional to the IGF1 concentration. A calibration curve is plotted and IGF1 concentration in samples is determined by interpolation from the calibration curve.

Catalog # KAR1581
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 100 µL
Controls 2 levels
Range 15-774 ng/mL
Sensitivity 4,5 ng/mL
Incubation 1h/15 min. at RT with shaking at 500 rpm
Shelf Life (weeks) 96
Calibration 1 ng = 1 ng of the NIBSC 1st WHO IS 02/254

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1581.pdf
Download "Material Safety Data Sheet" MSKAP1581.pdf
Download catalogue "ELISA" CATE.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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