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CT, ELISA, Ultra Sensitive, 96 tests, For Research Use Only

The DIAsource CT-U.S.-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human CT – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the Calcitonin concentration.A calibration curve is plotted and Calcitonin concentration in samples is determined by interpolation from the calibration curve.

Catalog # KAR0421
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 100 µL
Controls 2 levels
Range 10-400 pg/mL
Sensitivity 0,7 pg/mL
Incubation Overnight at 2-8°C
Shelf Life (weeks) 48
Calibration 1 pg = 0.19µIU NIBSC 89/620

For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP0421.pdf
Download "Material Safety Data Sheet" MSKAP0421.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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