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PTH Intact, IRMA, 96 tests, For Research Use Only

DIAsource hPTH-120 min-IRMA is a two-step immunoradiometric assay based on coated-tube separation. It allows the determination of intact human PTH (hPTH) in serum. Goat antibodies specific to the 1-34 hPTH fragment (N-terminal fragment) are attached to the lower and inner surface of the plastic tubes. Calibrators or samples are added to the tubes. After 1 hour incubation, washing removes the occasional excess of antigen, mid-regional and C-terminal fragments. 125I labelled monoclonal antibodies specific to the 44-68 hPTH fragment are added. After 1 hour incubation and washing the remaining radioactivity bound to the tube reflects the intact h-PTH concentration. This two-step IRMA is highly specific of the intact h-PTH and does not cross react with active and inactive fragments even at high concentrations as suggested by HACKENG and al.

Catalog # KIR1491
Format IRMA
Label I125
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 300 µL
Controls 2 levels
Range 13-1562 pg/mL
Sensitivity 1,7 pg/mL
Incubation 2h at RT with shaking
Shelf Life (weeks) 9
Calibration 1 pg = 1 pg of NIBSC 95/646

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KIP1491.pdf
Download "Material Safety Data Sheet" MSKIP1491.pdf
Download catalogue "RIA" CATR.pdf
Download RIA Availability Calendar CALRIA21d.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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