Anti-HCV Elisa V 4.0 adopts the "direct sandwich principle" as the basis for the assay to detect antibodies to Hepatitis C virus (anti-HCV). It is a forth generation enzyme immunoassay kit, which uses recombinant HCV antigens (Core, NS3 and NS5 antigens) for the detection of Antibody to Hepatitis C virus (anti-HCV) in human serum or plasma. *1-3. These antigens, which are reactive with the predominant antibodies of HCV, constitute the solid phase antigenic absorbent. When human serum or plasma is added to the well, the HCV antigens and Anti-HCV will form complexes on the wells if Anti-HCV is present in the specimen. The wells are washed to remove the unbound materials. The diluted HCV Ag•HRPO Conjugate is added to the well and results in the formation of (HCV) • (Anti-HCV) • (HCV Ag•HRPO) complex. After washing out the unbound conjugate, TMB substrate solution is added for color development. The intensity of color development is proportional to the amount of antibodies present in the specimen.
Catalog # | KAPG4NAE3 |
Format | ELISA |
Label | HRP |
Size | 96 tests |
Sample Type | Serum, Plasma |
Sample Volume | 100 µL |
Controls | 2 levels |
Incubation | 60 min+30 min+ 15 min |
Calibration | NIBSC 02/238-004 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.