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HBV, HBsAg Screening, ELISA, 96 tests

HBsAg Elisa is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay ) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg, whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs•peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg boud to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 620 to 690nm.

Catalog # KAPG4SGE3
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 50 µL
Controls 2 levels
Sensitivity 0.2 ng/mL
Incubation 60 min/ 30 min
Calibration NIBSC 01/476-006

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPG4SGE3.pdf
Download "Material Safety Data Sheet" MSKAPG4SGE3.pdf
Download catalogue "ELISA"
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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