The radioimmunoassay of dehydroepiandrosterone (DHEA) is a competition assay. Prior to the assay, samples (serum or plasma) are extracted with ethyl ether; the solvent is evaporated and the dry residues are re-dissolved in the recovery buffer of the kit. The re-dissolved extracts and calibrators are then incubated with 125I-labeled DHEA,as tracer in antibody-coated tube. After incubation, the content of tubes is aspirated and the bound radioactivity is determined. A calibration curve is established and unknown values are determined by interpolation from the curve.
Catalog # | KIPB1138 |
Format | RIA |
Label | I125 |
Size | 100 tests |
Sample Type | Serum, Plasma |
Sample Volume | 300 µL |
Range | 0,5-35 ng/mL |
Sensitivity | 0,17 ng/mL |
Incubation | 1h at 37°C |
Shelf Life (weeks) | 8 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.